Cell Based Assays
Promega ROCK2 Kinase Enzyme System, 1mg
ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element.
Promega HDAC-Glo™ I/II Assay and Screening Systems
The HDAC-Glo™ I/II Assays and Screening System are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes from cells, extracts or purified enzyme sources.
Promega Glucose Uptake-Glo Assay, 5ml
The Glucose Uptake-Glo Assay is a non-radioactive, plate-based, homogeneous bioluminescent method for measuring glucose uptake in mammalian cells based on the detection of 2-deoxyglucose-6-phosphate (2DG6P).
Promega ERK1 Kinase Enzyme System, 1mg
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega WNK1 Kinase Enzyme System, 1mg
Recombinant human WNK1 (181-507) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + IKKβ Kinase Enzyme System
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega FMS Kinase Enzyme System, 1mg
Recombinant human FMS (539-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FMS is a proto-oncogene that encodes the tyrosine kinase transmembrane receptor for colony stimulating factor 1 receptor (CSF1R).
Promega Corporation Triglyceride-Glo Assay
Detect Triglyceride from a variety of biological samples works with lysed cells, tissues, medium, and serum samples. No organic extraction, extreme heat, or centrifugation required, with linear triglyceride detection up to 80 μM.
Promega T Cell Activation Bioassay (NFAT), 1 each
The T Cell Activation Bioassays are bioluminescent cell-based assays that overcome the limitations of existing assays and can be used for the discovery and development of novel biologics such as bispecific antibodies and CAR-T cell therapies.
Promega AKT1 Kinase Enzyme System, 1mg
Recombinant full-length human AKT1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKB alpha is a serine/threonine kinase that belongs to the AKT family.
Promega ADP-Glo™ Kinase Assay + AMPK (A2/B1/G1) Kinase Enzyme System
Recombinant full-length human AMPK (A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for diabetes treatment.
Promega InsR Kinase Enzyme System, 1mg
Recombinant human InsR (amino acids 1011-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. InsR is the insulin receptor tyrosine kinase that is involved in insulin signaling.
Promega EIF2AK2 Kinase Enzyme System, 1mg
Recombinant human EIF2AK2 (252-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EIF2AK2 (also known as double-stranded RNA-activated protein kinase) is shown to be involved in HIV/gp120-associated neurodegeneration.
Promega ADP-Glo™ Kinase Assay + SIK Kinase Enzyme System
Recombinant human SIK (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. SIK is a protein kinase that is involved in regulating AMPK-related kinases.
Promega ADP-Glo™ Kinase Assay + PKCμ Kinase Enzyme System
Recombinant full-length human PKC mu was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC mu is a member of the protein kinase C family that differs from the other PKC isoenzymes in structural and enzymatic properties.